ypgal media  (Vector Laboratories)

Journal: The EMBO Journal

Article Title: Multifunctional roles of Brl1-Brr6 in nuclear envelope fusion during nuclear pore complex biogenesis

doi: 10.1038/s44318-026-00718-y

Figure Lengend Snippet: ( A ) AαH predictions for the wild-type and mutant sequences of Brr6 were generated using HeliQuest (Gautier et al, ). The upper cartoon illustrates the domain organization of Brr6, highlighting the two transmembrane (TM) domains, the AαH, and the four conserved cysteine residues. Amino acid positions are indicated. ( B ) Subcellular localization of GFP-tagged Brr6 wild-type and brr6 L145E AαH region. The pGal1 promoter was expressed for 3 h by the addition of galactose. Fluorescence microscopy was performed to assess localization. dsRed-HDEL was used as NE and ER marker (Madrid et al, ). Size bar: 5 µm. Representative image from three independent repeats. ( C ) Growth assay of the BRR6 shuffle strain carrying the empty LEU2 -based plasmid pRS315 or pRS315 containing the indicated BRR6 alleles ( BRR6 , brr6 L145E , and brr6 F152E ). Tenfold serial dilutions were spotted onto SC–LEU and 5-FOA plates and incubated at 30 °C for 2 days. Growth on 5-FOA plates selects for cells that have lost the URA3 plasmid, thereby testing whether the LEU2 -based plasmid alone can support viability in the absence of wild-type BRR6 . One representative of three independent experiments is shown. ( D ) Wild-type yeast cells carrying the indicated pGal1 plasmids were spotted in tenfold serial dilutions onto glucose (Glu) or galactose/raffinose (Gal/Raf) plates and incubated at 30 °C. One representative of three independent experiments is shown.

Article Snippet: Cells overexpressing these mutant alleles displayed severe growth defects on the inducing galactose plates (Gal/Raf) while control cells (pGal1- BRR6 and pGal1 vector) showed robust growth (Fig. ).

Techniques: Mutagenesis, Generated, Fluorescence, Microscopy, Marker, Growth Assay, Plasmid Preparation, Incubation

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Identification of glycan structures on total hemocytes from Cherax quadricarinatus by immunofluorescence and flow cytometry using lectin staining. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ). Representative scatter plot shows internal complexity (side scatter, SSC) and positive for lectin staining. Immunofluorescences are representative for each lectin staining. Scale bar: 10 µm.

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Immunofluorescence, Flow Cytometry, Staining

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Identification of glycan structures by subpopulation of hemocytes from Cherax quadricarinatus by Flow cytometry using lectin staining. Value in each histogram represent mean of percentage of three independent experiments. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA-I (from Sambucus nigra ), MAA-I ( Maackia amurensis ). Red lines represent control assays for specificity recognition using lectins incubated previously with 200 mM specific carbohydrates 30 min before the assays, blue lines are lectins without inhibition.

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Flow Cytometry, Staining, Control, Incubation, Inhibition

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Glycan patterns by subpopulation hemocytes after immunostimulation. raw starch microparticles (SMP), Vibrio parahaemolyticus (Vp) and Escherichia coli (Ec). Value in each heatmap represent mean of percentage of three independent experiments. Significant differences are mentioned in the text. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ).

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Starch

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Identification of glycan structures on total hemocytes from Cherax quadricarinatus by immunofluorescence and flow cytometry using lectin staining. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ). Representative scatter plot shows internal complexity (side scatter, SSC) and positive for lectin staining. Immunofluorescences are representative for each lectin staining. Scale bar: 10 µm.

Article Snippet: C2010, St Louis, MO, USA); LTL from Lotus tetragonolobus (specificity: Fuc α1–3/−2 (Gal β1–4) GlcNAc) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Immunofluorescence, Flow Cytometry, Staining

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Identification of glycan structures by subpopulation of hemocytes from Cherax quadricarinatus by Flow cytometry using lectin staining. Value in each histogram represent mean of percentage of three independent experiments. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA-I (from Sambucus nigra ), MAA-I ( Maackia amurensis ). Red lines represent control assays for specificity recognition using lectins incubated previously with 200 mM specific carbohydrates 30 min before the assays, blue lines are lectins without inhibition.

Article Snippet: C2010, St Louis, MO, USA); LTL from Lotus tetragonolobus (specificity: Fuc α1–3/−2 (Gal β1–4) GlcNAc) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Flow Cytometry, Staining, Control, Incubation, Inhibition

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Glycan patterns by subpopulation hemocytes after immunostimulation. raw starch microparticles (SMP), Vibrio parahaemolyticus (Vp) and Escherichia coli (Ec). Value in each heatmap represent mean of percentage of three independent experiments. Significant differences are mentioned in the text. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ).

Article Snippet: C2010, St Louis, MO, USA); LTL from Lotus tetragonolobus (specificity: Fuc α1–3/−2 (Gal β1–4) GlcNAc) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Starch